BIO 230 - MICROBIOLOGY

SPRING SEMESTER - 1999-2000

Date Lecture Chapter Lab No.

Jan. 20 R Intro., Microbial Hist., Meth. 1, 2

25 T Prokaryotic Cell Structure 3 1, 2, 3, 5

27 R Prokaryotic Cell Structure 3

Feb. 1 T Prokaryotic Cell Structure 3 6, 7, 8, 9

3 R Eukaryote Cells 4

8 T Microbial Nutrition 5 10, 11, 12, 13

10 R Microbial Growth 6

15 T Microbial Growth 6 14, 15, 16

17 R Control of Microorganisms 7

22 T EXAM I 17, 18, 20

24 R Control of Microorganisms 7

29 T Metabolism- Gen. of ATP 8, 9 21, 22, 24, 25

Mar. 2 R Metabolism: Biosynth. 10

7 T Metabolism: Biosynth. 10, 11 23, 27, 28

9 T Reg. of Enzyme Activity 12 conclude above+notebooks

SPRING RECESS BEGINS 5:00 P.M. MARCH 10 & ENDS 8:00 A.M. MARCH 20

21 T Microbial Genetics 13, 14 40, 41(unknown), 79

23 R Viruses: General 16

28 T Bacteriophages 17 41, 57

30 R EXAM II

Apr. 4 T Viruses of Eucaryotes 18 41, 57

6 R Microbial Taxonomy

11 T Archaea 20 41(conclude), Biolog, 57 (conclude)

13 R Proteobacteria 22

18 T Symbiotic Associations 28, 29 Biolog, 52

20 R Antimicrobial Chemotherapy 33

EASTER RECESS BEGINS 5:00 P.M. Thurs., APRIL 21 & ENDS 8:00 A.M., Tues., APRIL 25

25 T Epidemiology 35 48, 54, 55

27 R Food Microbiology 43 Unknown and Biolog report due

May 2 T Industrial Microbiology 44 lab cleanup

4 R Follow Friday Schedule Phage Report Due, Notebooks due

READING DAY: FRIDAY MAY 5 FINAL EXAM: WEDNESDAY, MAY 10, 1:30-4:30 P.M.

BIO 230 - MICROBIOLOGY, SPRING, 2000

Course Grading

2 Lecture exams at 20% each = 40%

5 Lecture/lab quizzes at 2% each = 10%

General unknown report 10%

2 Lab notebook reviews at 5% = 10%

Phage Lab project (pairs) - due May 4 10%

Final exam 20%

Total 100%

Textbook

Prescott, L. M., et al., 1999. Microbiology, 4th ed. W.C. Brown.

Lab Manual

Harley, J.P. and L.M. Prescott. 1999. Laboratory Exercises in Microbiology, 4th ed. W.C. Brown.

Laboratory Notes

In order to gain the most from the laboratory experience, you must read the background information and techniques of the exercises to be done that day before coming to the lab. Not knowing what is expected is detrimental to you and those with whom you work. Unannounced quizzes will be given at the beginning of some labs. All laboratory work done in pairs or groups for this course is to be considered one's own once the data has been collected. Inferences or conclusions drawn from the data and entered into the lab manual are not the group's but your own. THINK FOR YOURSELF!

As a part of the laboratory experience, occasionally you will be required to observe the results of inoculations made during the scheduled lab one or two days after on your own schedule or in class. This is especially true for your unknowns and projects. Please make every effort to do so during the day so that I may be available to answer questions or assist with problems.

Your laboratory project will be carried out during the last few weeks of the course. It will consist of purifying and characterizing a bacteriophage isolate from a natural source (sewage). Lab manual exercise 57 can be used as a guide for this project. However, you will be responsible for protocol planning, media and equipment preparation, searching for pertinent literature, and performing the experiments necessary to perform the project adequately. Minimally, you will provide a pure, known, high-titer phage suspension, characterize and measure the plaque form, and determine host range using a limited number of E. coli and other enteric species. If time permits, we will try to visualize your phage using the TEM and the negative staining technique. A formal paper written in standard scientific format will be due at the end of the semester. The paper is to have references from the primary and review literature and may include a web site or two. Members of the team will receive the same grade unless one does the brunt of the work and the other does not.

Supplemental Readings