SEM Procedure
To prepare specimens for the SEM, they are first fixed
with Karnovsky's fixative and then taken through a graded alcohol dehydration
series. Once dehydrated, the specimens are placed in a critical point dryer
(left image), mounted and placed in a gold coater (center image). Once
gold coating is complete, specimens are ready to be viewed on the SEM.
Images may be scanned on a digital imaging system by computer enhancement
(right image) or polaroid pictures may be taken using an attached
camera.
Back to SEM Page
