Testcross

An individual who expresses the dominant phenotype for a given gene can be either homozygous (AA) or heterozygous (Aa). A testcross is performed to determine the genotype of the dominant phenotype individual. The individual in question is crossed to a tester (homozygous for the recessive allele - genotypically aa).

Possibility 1: individual is AA

    A cross of AA X aa would produce progeny that are all Aa genotypically and all progeny would express the dominant phenotype.
Possibility 2: individual is Aa
    A cross of Aa X aa would produce progeny such that 1/2 would be of Aa genotype (dominant phenotype) and 1/2 would be of aa genotype (recessive phenotype).
Composition of progeny phenotype allows you to determine if the individual with the dominant phenotype is AA or Aa.